Trypsin
Trypsin is a naturally occurring proteolytic enzyme found in the digestive tract of mammals. It is a serine protease that plays a critical role in protein hydrolysis and nutrient absorption. Activated from its inactive precursor, trypsinogen, trypsin functions as an active peptide hydrolase, specifically cleaving peptide chains at the carboxyl side of lysine and arginine residues.
Description
Biolaxi Enzymes is a manufacturer and supplier of Trypsin enzymes, designed for a wide range of industrial and biomedical applications. Our Trypsin enzymes are produced under strict quality control protocols, ensuring purity, stability, and consistent performance. We serve industries such as pharmaceuticals, biotechnology, food processing, and research, delivering reliable and innovative Trypsin solutions tailored to meet specific operational requirements.
Our Trypsin enzymes are essential in pharmaceuticals and biotechnology for applications like cell dissociation and protein digestion, in food processing for protein hydrolysis, and in research for precise protein analysis.
Clinically, our Trypsin is used in combination with bromelain and rutosides to treat certain inflammatory conditions. It is also utilized as a wound-healing agent, including for mouth ulcers, where it effectively removes dead tissue and promotes healthy tissue growth. The combination of Trypsin and chymotrypsin has proven to be more effective in addressing inflammatory symptoms and supporting recovery from severe tissue injuries.
Technical Specifications
| Parameters | Details |
| CAS Number | 9002-07-7 |
| EC Number | 3.4.21.4 |
| Brand | Biolaxi Enzymes Pvt. Ltd. |
| Activity | ≥ 2,500 USP units/mg |
| Purity | ≥ 90% (SDS-PAGE) |
| pH Stability | 7.0 - 9.0 |
| Temperature Stability | 25°C - 50°C |
| Form | Lyophilized powder or liquid formulation |
| Storage | 2°C - 8°C (Dry and protected from moisture) |
| Solubility | Soluble in water and buffer solutions |
| Endotoxin Levels | < 0.1 EU/mg (For cell culture applications) |
Trade Information
| Supply Ability | 20000 Kilograms Per Month |
| Delivery Time | 10 Days |
| Sample Available | Free samples are available |
| Minimum Order Quantity | 5 kg, 25 kg, 50 kg |
| Packaging | PVC bags/bottles/Drums or in the aluminium pouch. |
| Main Export Markets | Australia, North America, South America, Central America, Western Europe, Eastern Europe, Middle East, Asia, Africa |
| Main Domestic Market | All India |
Mechanism
Trypsin is a serine protease that catalyzes the hydrolysis of peptide bonds, specifically cleaving at the carboxyl side of lysine (K) and arginine (R) residues. This proteolytic activity breaks down complex proteins into smaller peptides or amino acids, facilitating digestion and protein analysis. The enzyme operates optimally in alkaline conditions (pH 7.0 – 9.0) and requires calcium ions for stability and activity. Its mechanism involves a catalytic triad (serine, histidine, and aspartate) that enables precise and efficient protein cleavage.
Isozymes
Trypsin enzymes exist in multiple isozyme forms, each with slight variations in structure and function. These isozymes are categorized based on their source and specific activity profiles:
Trypsin-1 (Cationic Trypsin):
- Predominant form found in the pancreas.
- High stability and activity in alkaline environments.
- Commonly used in biotechnological applications due to its robust performance.
Trypsin-2 (Anionic Trypsin):
- Less abundant but exhibits higher catalytic efficiency in certain conditions.
- Often used in specialized research applications requiring precise protein digestion.
Trypsin-3 (Mesotrypsin):
- A minor variant with unique substrate specificity.
- Resistant to natural protease inhibitors, making it valuable in specific diagnostic and therapeutic applications.
Applications
Pharmaceuticals and Biotechnology:
- Cell Culture: Used for dissociating adherent cells from culture surfaces, enabling subculturing and downstream analysis.
- Protein Digestion: Facilitates the breakdown of proteins for peptide mapping and mass spectrometry analysis.
- Drug Development: Supports the production of biologics and vaccines by processing recombinant proteins.
Food Industry:
- Protein Hydrolysis: Enhances the digestibility and nutritional value of protein-rich foods, such as infant formula and sports nutrition products.
- Flavor Enhancement: Breaks down proteins to release amino acids, improving flavor profiles in processed foods.
Research and Diagnostics:
- Proteomics: Essential for protein identification and characterization in research studies.
- Diagnostic Kits: Used in immunoassays and diagnostic tests for disease detection and biomarker analysis.
Industrial Applications:
- Leather Processing: Aids in dehairing and bating during leather production.
- Waste Management: Breaks down proteinaceous waste in environmental cleanup processes.
Therapeutic and Clinical Uses:
- Inflammatory Conditions: Used in combination with bromelain and rutosides to treat inflammation.
- Wound Healing: Applied directly to wounds, including mouth ulcers, to remove dead tissue and promote healthy tissue growth.
Certificates
- Halal Certificate
- ISO 9001:2015
- cGMP
- Food and Drugs Administration
- GMP Certificate
- Kosher Certificate
Contact Details
| Phone No. | +91 9158047802 |
| support@biolaxienzymes.com | |
| Location | Thane West, Maharashtra - 400615 |